HeLa transfection with plasmid DNA using DreamFect reagent

3x10^6 HeLa cells seeded in 15 cm diameter dishes were transfected with 5 µg plasmid DNA using 40 µL DreamFect transfection reagent.

This article demonstrates the high efficiency of DreamFect from OZ Biosciences for DNA transfection into HeLa cells.

article reference: Bio-protocol 4(7): Vol 4, Iss 7, 4/5/2014

UPF1 RNA Immunoprecipitation from Mini-μ Construct–expressing Cells

David Zünd and Oliver Mühlemann
 
Abstract
UPF1, an RNA helicase and a core factor of nonsense-mediated mRNA decay (NMD), interacts with RNA independently of the sequence context. To investigate the influence of translation on the association of UPF1 with specific reporter transcripts, UPF1 RNA immunoprecipitations (RIPs) are performed from Hela cells that either express a normally translated immunoglobulin-µ (Ig-µ) reporter (mini µ) or a version with a stable stem loop in the 5' UTR (SL mini µ) that efficiently inhibit translation initiation (Zund et al., 2013). Both the cloning of the SL mini µ reporter construct and the UPF1 RIP experiment are described in detail.

DreamFect from OZ Biosciences allows transfecting all types of nucleic acids with a very high efficiency; it is fully biodegradable and does not interfere with cellular mechanisms.