Use of #Lullaby to safely transfect #siRNA and screen Malignant Rhabdoid Tumors

Dual Targeting of PDGFR and FGFR1 Displays Synergistic Efficacy in Malignant Rhabdoid Tumors

Cell, Oct 2016 
Wong et al.

Abstract
Malignant rhabdoid tumors (MRTs) are pediatric cancers characterized by a deficiency in the SWI/SNF subunit SMARCB1. Wong et al. show that MRTs display coactivation of PDGFR and FGFR1 and that dual blockade of these receptors induces apoptosis. These findings present therapeutic opportunities to exploit tyrosine kinase dependencies in cancers with SWI/SNF  deficiencies.

siRNA transfections were performed as follows, 2000 cells/well were reverse transfected in 96-well plates with SMARTpool siRNAs (Dharmacon) using Lullaby reagent (Oz Biosciences). Where indicated, cells were treated with vehicle or drug 24h post transfection. Apoptosis and cell viability were measured using Caspase 3/7 Glo and Cell Titre Glo (Promega), respectively, 72-96h post transfection according to manufacturer’s instructions and normalised to cells transfected with a non-targeting siRNA pool.