Human gastric cell line N87 transduction by shRNA using ViroMag R/L.

N87 cell line (human gastric) were transduced by shRNA-carrying lentiviral praticles using ViroMag R/L beads.

This article highlights the ability of ViroMag R/L (OZ Biosciences) to enhance infection and transduction capacities of lentiviral particles in N87 cell line.

paper reference: PLoS One. 2013;8(1):e53540. doi: 10.1371/journal.pone.0053540. Epub 2013 Jan 4.

Musashi-1 Post-Transcriptionally Enhances Phosphotyrosine-Binding Domain-Containing m-Numb Protein Expression in Regenerating Gastric Mucosa.
Takahashi T, Suzuki H, Imai T, Shibata S, Tabuchi Y, Tsuchimoto K, Okano H, Hibi T.

Abstract

OBJECTIVE:
Upregulation of the RNA-binding protein Musashi-1 (Msi1) has been shown to occur in rat gastric corpus mucosa after ethanol-induced mucosal injury. However, there is no direct evidence linking Msi1 with gastric regeneration. We examined the process of tissue repair after acute gastric mucosal injury with Msi1-knock-out (KO) mice to clarify the role of Msi1 and Msi1-dependent regulation of m-Numb expression in regenerating gastric mucosa.
METHODS:
Acute gastric injury was induced in Msi1-KO and wild-type ICR mice by administering absolute ethanol. Expression of the splicing variants of m-Numb mRNA and protein in the gastric mucosa were analyzed by quantitative RT-PCR and western blotting, respectively.
RESULTS:
We demonstrated that phosphotyrosine-binding domain-containing m-Numb expression was significantly upregulated at both the mRNA and protein levels in wild-type mice at 3 h after ethanol-induced acute gastric injury. In contrast, in Msi1-KO mice, the m-Numb protein was expressed weakly, and was associated with delayed regeneration of the injured gastric mucosal epithelium. In the Msi1-KO mouse, the ratio of m-Numb mRNA to total m-Numb mRNA in the heavy polysome fractions was lower than that in the wild-type mouse. Further, we showed that m-Numb-enhancement in gastric mucous cells induced the expression of prostate stem cell antigen and metallothionein-2. Under the m-Numb enhancing condition, the gastric cells exhibited enhanced cell proliferation and were significantly more resistant to H(2)O(2)-induced cell death than control cells.
CONCLUSIONS:
Msi1-dependent post-transcriptional enhancement of m-Numb is crucial in gastric epithelial regeneration.

ViroMag R/L from OZ Biosciences is a magnetic nanoparticles formulation optimized for lentiviruses and retroviruses dedicated to increase virus infection in vitro and in vivo.