NCI-H441 transfection and Jurkat T cells transfection with DreamFect Gold(TM) and rotational magnetofection

Magnetic lipoplexes were prepared with DreamFect Gold lipid-based transfection reagent and control plasmids.

This paper demonstrates the efficiency of magnetofection (rotational) associated with DreamFect Gold to transfect human pulmonary epithelial cells derived from a papillary carcinoma of the lung and Jurkat T cells.

Paper reference: Journal of Magnetism and Magnetic Materials

Rotational magnetic pulses enhance the magnetofection efficiency in vitro in adherent and suspension cells
Ch. Dahmani, O. Mykhaylyk,Fl. Helling,St. Götz,Th. Weyh,H.-G. Herzog,Ch. Plank

Abstract

The association of magnetic nanoparticles with gene delivery vectors in combination with the use of gradient magnetic fields (magnetofection) enables improved and synchronised gene delivery to cells. In this paper, we report a system comprising rotating permanent magnets to generate defined magnetic field pulses with frequencies from 2.66 to 133 Hz and a field amplitude of 190 or 310 mT at the location of the cells. Low-frequency pulses of 2.66–10 Hz with a magnetic flux density of 190 mT were applied to the examined cells for 30–120 s after magnetofection. These pulses resulted in a 1.5–1.9-fold enhancement in the transfection efficiency compared with magnetofection with only a static magnetic field in both adherent and suspension cells. The magnetic field amplitudes of 190 and 310 mT had similar effects on the transfection efficacy. No increase in the percentage of transgene-expressing suspension cells and no cytotoxic effects (based on the results of the MTT assay) were observed after applying alternating magnetic fields.

 magnetic fields.

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Highlights

► We developed a magnetic system capable of generating defined magnetic pulses based on permanent magnets. ► The main advantage of the system is the lack of heat-induced fluctuations in the working parameters. ► Our system succeeded in enhancing the transfection of adherent human lung epithelial cells and human suspension cells. ► The enhancement in the transfection efficiency compared with static magnetic field is due to the magnetic field pulses. ► The approach could be used as a complementary method for drug targeting

Keywords

• Magnetic nanoparticles;
• Transfection;
• Magnetofection;
• Pulsed magnetic field;
• Non-viral vectors;
• Nucleic acid delivery

DreamFect Gold from OZ Biosciences allows transfecting all types of nucleic acids with a very high efficiency; it is fully biodegradable and does not interfere with cellular mechanisms.