U-87 MG, Hep G2, MDA-MB-231 transfection with DNA using PolyMag

PolyMag transfection reagent was used as a base for cationic lipid-mediated transfection in several human cancer cells. 

This paper shows the high efficiency of PolyMag transfection reagent from OZ Biosciences to transfect several human cancer cell lines. 

article reference: Int J Pharm. 2013 Feb 13. pii: S0378-5173(13)00106-3. doi: 10.1016/j.ijpharm.2013.01.055.

Gene delivery into human cancer cells by cationic lipid-mediated magnetofection.

Govindarajan S, Kitaura K, Takafuji M, Ihara H, Varadarajan KS, Patel AB, Gopal V.

Abstract

In this study, a combination of magnetic nanoparticles (MNPs) with ethyl or methyl functional groups together with cationic lipid N,N-di-n-hexadecyl-N,N-dihydroxyethylammonium chloride formulated with colipid cholesterol, upon magnetofection, enhanced DNA uptake into human glioblastoma-astrocytoma, epithelial-like cell line U-87 MG, hepatocellular carcinoma HepG2, cervical cancer HeLa and breast cancer MDA-MB-231 cells. Having confirmed this, we monitored uptake of plasmid DNA mediated by ternary magnetoplexes by fluorescence microscopy, flow cytometry and reporter gene expression assays in the presence and absence of a magnetic field. Our observations clearly indicate enhanced transfection efficiency in vitro, upon magnetofection, in the presence of serum as seen from β-Gal reporter gene expression. The observed activity in serum suggests the suitability of MNPs for in vivo applications. Further, we measured the transverse relaxation time (T(2)) and obtained T(2)-weighted MRI images of treated U-87 MG cells. T(2) determined for MNP-VP-Me(22) and MNP-VP-Et(22) corresponds to 22.6±0.8ms and 36.0±2.1ms, respectively, as compared to 47±1.7ms for control, suggesting their applicability in molecular imaging. Our results collectively highlight the potential of lipid-based approach to augment magnetic-field guided-gene delivery using MNPs and additionally towards developing intracellular molecular probes for MRI-based imaging.
Copyright © 2013 Elsevier B.V. All rights reserved.

The universal and powerful PolyMag formulation allows you to achieve very high transfection efficiency. It is based on Magnetofection™ transfection technology. PolyMag reagent has been optimized to be used with all types of nucleic acids.