Melanoma cells transfection (WM-266-4) with siRNA using PolyMag

Small interfering RNA (siRNA) were transfected into WM-266-4, melanoma cell line using Magnetofetion technology with PolyMag transfection reagent.

This paper shows the high efficiency of PolyMag transfection reagent from OZ Biosciences to transfect siRNA in melanoma cell line.

paper reference: Sci Rep. 2013 Apr 16;3:1661.

Xentry, a new class of cell-penetrating peptide uniquely equipped for delivery of drugs.
Montrose K, Yang Y, Sun X, Wiles S, Krissansen GW.

Abstract

Here we describe an entirely new class of cell-penetrating peptide (CPP) represented by the short peptide Xentry (LCLRPVG) derived from an N-terminal region of the X-protein of the hepatitis B virus. Xentry permeates adherent cells using syndecan-4 as a portal for entry, and is uniquely restricted from entering syndecan-deficient, non-adherent cells, such as resting blood cells. Intravenous injection of Xentry alone or conjugated to β-galactosidase led to its delivery to most tissues in mice, except circulating blood cells. There was a predilection for uptake by epithelia. Anti-B-raf antibodies and siRNAs linked to Xentry were capable of killing B-raf-dependent melanoma cells. Xentry represents a new class of CPP with properties that are potentially advantageous for life science and therapeutic applications.

The universal and powerful PolyMag formulation allows you to achieve very high transfection efficiency. It is based on Magnetofection™ transfection technology. PolyMag reagent has been optimized to be used with all types of nucleic acids.