Total protein levels measurement in BALF supernatants of Mice using the Bradford Protein Assay Kit from OZ Biosciences

Measurement of protein level in Bronchoalveolar lavage fluid (BALF)  of mice right lung was  performed using Bradford Protein Assay Kit (B-Pak) from OZ Biosciences. 

This article demonstrate the capacity of the Bradford Protein Assay Kit from OZ Biosciences  to efficiently measure the total protein concentration in BALF of mice lung.

paper reference: Critical Care Research and Practice, vol. 2013, Article ID 435236, 11 pages, 2013. doi:10.1155/2013/435236

The Extent of Ventilator-Induced Lung Injury in Mice Partly Depends on Duration of Mechanical Ventilation
Maria A. Hegeman, Sabrine N. T. Hemmes, Maria T. Kuipers, Lieuwe D. J. Bos, Geartsje Jongsma, Joris J. T. H. Roelofs, Koenraad F. van der Sluijs, Nicole P. Juffermans, Margreeth B. Vroom, and Marcus J. Schultz

Abstract

Background. Mechanical ventilation (MV) has the potential to initiate ventilator-induced lung injury (VILI). The pathogenesis of VILI has been primarily studied in animal models using more or less injurious ventilator settings. However, we speculate that duration of MV also influences severity and character of VILI. Methods. Sixty-four healthy C57Bl/6 mice were mechanically ventilated for 5 or 12 hours, using lower tidal volumes with positive end-expiratory pressure (PEEP) or higher tidal volumes without PEEP. Fifteen nonventilated mice served as controls. Results. All animals remained hemodynamically stable and survived MV protocols. In both MV groups, PaO2 to FiO2 ratios were lower and alveolar cell counts were higher after 12 hours of MV compared to 5 hours. Alveolar-capillary permeability was increased after 12 hours compared to 5 hours, although differences did not reach statistical significance. Lung levels of inflammatory mediators did not further increase over time. Only in mice ventilated with increased strain, lung compliance declined and wet to dry ratio increased after 12 hours of MV compared to 5 hours. Conclusions. Deleterious effects of MV are partly dependent on its duration. Even lower tidal volumes with PEEP may initiate aspects of VILI after 12 hours of MV.

The Bradford Protein Assay Kit (B-Pak) from OZ Biosciences is a straightforward and rapid procedure for determining the concentration of protein in solution.
The B-Pak is based on the binding of Coomassie Brilliant Blue G-250 dye to the proteins and particularly basic and aromatic amino acids residues. The dye exists in three forms: cationic (red), neutral (green) and anionic (blue).