Measurement of the total protein levels in BALF supernatants of Mice using the Bradford Protein Assay Kit from OZ Biosciences

Protein level in Bronchoalveolar lavage fluid (BALF) of mice right lung was measured using Bradford Protein Assay Kit (B-Pak) from OZ Biosciences. 

This article demonstrate the capacity of the Bradford Protein Assay Kit from OZ Biosciences  to efficiently measure the total protein concentration in BALF of mice lung.

paper reference: Shock. 2013 Jul 12.

High-Dose Acetylsalicylic Acid is Superior to Low Dose as Well as to Clopidogrel in Preventing LPS-Induced Lung Injury in Mice.
Tuinman PR, Muller MC, Jongsma G, Hegeman MA, Juffermans NP.

Abstract

BACKGROUND: Use of aspirin (ASA) was found to improve outcome in animal models of acute lung injury (ALI) or its more severe form ARDS. In ARDS patients, data indicating a protective effect of ASA are less convincing. We hypothesize that ASA in a high dose is superior to low dose ASA in preventing lung injury. Also, the effect on lung injury of inhibiting platelet activation by clopidogrel was investigated.
METHODS: ALI was induced by intranasal instillation of 10 μg lipopolysaccharide (LPS). Before LPS, BALB/c mice were pre-treated with either high dose of ASA (100 μg/g intraperitoneally (i.p.), low dose ASA (12.5 μg/g i.p), clopidogrel (50 μg/g i.p) or clopidogrel in combination with low dose of ASA. Controls received vehicle or LPS without intervention. Five hours after LPS, bronchoalveolar lavage fluid (BALF) and plasma were obtained.
MEASUREMENTS AND MAIN RESULTS: All treatment regimes reduced neutrophil influx in the BALF compared to LPS controls (high dose ASA 75±2 (mean±SD), low dose ASA 86±3, clopidogrel 82±1 and low dose ASA-clopidogrel 82±3 vs LPS control 88±2%, P ≤0.05). High dose ASA reduced BALF levels of protein compared to LPS controls (0.2[15] (median [IQR]) vs 75[20] pg/mL, P < 0.01), to a greater extent than after low dose ASA (48[32] pg/mL), clopidogrel (37[23]pg/mL) or low dose ASA-clopidogrel (57[8] pg/mL).
CONCLUSION: High dose ASA is superior to low dose ASA, clopidogrel and to a combination of clopidogrel and low dose ASA in attenuating LPS-induced lung injury in mice, suggesting high dose ASA to be the antiplatelet therapy of choice in further research on preventing ALI.

The Bradford Protein Assay Kit (B-Pak) from OZ Biosciences is a straightforward and rapid procedure for determining the concentration of protein in solution.
The B-Pak is based on the binding of Coomassie Brilliant Blue G-250 dye to the proteins and particularly basic and aromatic amino acids residues. The dye exists in three forms: cationic (red), neutral (green) and anionic (blue).