Transduction of C2C12 cells with MCL-retroviral particles using Magnetofection method.

C2C12 cells were transduced with MCL-retroviral particles using the Magnetofection method from OZ Biosciences.

This paper shows the efficiency of the Magnetofection technology from OZ Biosciences to enhance infection and transduction in C2C12 cells.

article reference: Biomaterials. 2010 Feb;31(6):1251-9.

Genetically engineered angiogenic cell sheets using magnetic force-based gene delivery and tissue fabrication techniques.
Akiyama H, Ito A, Kawabe Y, Kamihira M. 

Abstract

A major limitation in tissue engineering is the insufficient formation of blood vessels in implanted tissues, resulting in reduced cell density and graft size. We report here the fabrication of angiogenic cell sheets using a combination of two magnetic force-based techniques which use magnetite cationic liposomes (MCLs), magnetofection and magnetic cell accumulation. A retroviral vector encoding an expression cassette of vascular endothelial growth factor (VEGF) was labeled with MCLs, to magnetically attract the particles onto a monolayer of mouse myoblast C2C12 cells, for gene delivery. MCL-mediated infection increased transduction efficiency by 6.7-fold compared with the conventional method. During the fabrication of the tissue constructs, MCL-labeled cells were accumulated in the presence of a magnetic field to promote the spontaneous formation of a multilayered cell sheet. VEGF gene-engineered C2C12 (C2C12/VEGF) cell sheets, constructed using both magnetic force-based techniques, were subcutaneously transplanted into nude mice. Histological analyses revealed that on day 14 the C2C12/VEGF cell sheet grafts had produced thick tissues, with a high-cell density, and promoted vascularization. This suggests that the method described here represents a powerful strategy in tissue engineering.

OZ Biosciences provides several magnetic nanoparticles formulations optimized for increasing any kind of virus infection and transduction both in vitro and in vivo.