Endothélial Progenitor Cells (EPC) transfection with siRNA using LipoMag Kit from OZ Biosciences

Sub-confluent EPC were transfected with 50nmol/mL siRNA using LipoMag Kit (association of DreamFect Gold and CombiMag).

This article demonstrates the high efficiency of the association of Magnetofection (CombiMag) to Lipofection (DreamFect Gold) for siRNA transfection into endothelial progenitor cells - EPC.

article reference: Am J Physiol Heart Circ Physiol. 2013 Aug 9.

Apurinic/apyrimidinic Endonucelase 1 (APE1) Maintains Adhesion of Endothelial Progenitor Cells and Reduces Neointima Formation.
Yamauchi A, Kawabe JI, Kabara M, Matsuki M, Asanome A, Aonuma T, Ohta H, Takehara N, Kitagawa T, Hasebe N.

Abstract

Background; Apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that processes DNA-repair function and controls cellular response to oxidative stress. Endothelial progenitor cells (EPCs) are recruited to oxidative stress-rich injured vascular walls and positively contribute to vascular repair and endothelialization. We hypothesized that APE1 funcitons for EPCs-mediated inhibition of neointima formation in injured vasculature. Methods and Results; EPCs isolated from bone marrow cells of C57BL6 mice (12-16 weeks old) were able to survive in the presence of hydrogen peroxide (H₂O₂; up to 1000 μmole/l) due to the highly expressed ROS scavengers. However, adhesion capacity of EPCs was significantly inhibited by H₂O₂(100 μmole/l) even though an intracellular ROS was retained at small level. An APE1-selective inhibitor or RNA interference-mediated knockdown of endogenous APE1 in EPCs aggravated the H₂O₂-mediated inhibition of EPCs-adhesion. In contrast, when APE1 was overexpressed in EPCs using an adenovirus harboring the APE1 gene (APE-EPCs), adhesion was significantly improved during oxidative stress. To examine in vivo effects of APE1 in EPCs, APE-EPCs were transplanted via the tail vein after wire-mediated injury of the mouse femoral artery. The number of adherent EPCs at injured vascular walls and the vascular repair effect of EPCs were enhanced in APE-EPCs compared to control EPCs. Conclusion; Among the cellular functions of EPCs, adhesion is especially sensitive to oxidative stress. APE1 enhances in vivo vascular repair effects of EPCs in part through the maintenace of adhesion properties of EPCs. APE1 may be a novel and useful target gene for effective cellular transplantation therapy. 

This new kit is an association of DreamFect Gold and CombiMag reagents. DreamFect™ Gold formulation is a lipofection reagent achieving high transfection efficiency and high transgene expression level. CombiMag is a Magnetofection™ reagent: versatile and improving transfection efficiency without toxicity. The LipoMag Kit combines both of the advantages of lipofection and magnetofection transfection technique to transfect all kind of cells with the best efficiency!