Hippocampal neurons in 35mm dish were transfected using 7.0 µL Lipofectamine 2000 and 1.0 µL CombiMag, two transfection reagents associated in the Magnetofectamine Kit
This paper demonstrated the high efficiency of Magnetofectamine from
OZ Biosciences
to transfect primary hippocampal cultures.
article reference:Neurophysiology, May 2013, Volume 45, Issue 3, pp 193-199
Chlorpyrifos Leads to Oxidative Stress-Induced Death of Hippocampal Cells in Vitro
Yu. T. Salyha
Abstract:
Chlorpyrifos (ClPF) is a broad-spectrum organophosphate insecticide
widely used in agriculture, industry, and at home. Like all
organophosphates, ClPH affects the nervous system by inhibiting the
enzyme acetylcholinesterase (AChE). In addition, it is transformed in
higher animals into ClPF-oxon that is about 3000 times more potent
against the nervous system than ClPF itself. As was found recently, the
action on ACh is not the only mechanism of ClPF toxicity. One other
mechanism of this organophosphate is induction of oxidative stress
leading to generation of free radicals. We investigated the effects of
ClPF on hippocampal cells of the rat
in vitro
and focused our attention on mediation of its cytotoxic effect related
to the production of reactive oxygen species. Transfection of cultured
hippocampal cells by green fluorescent protein (GFP) was used. We
studied the dose dependence of the intensity of ClPF-induced damage and
cell death of hippocampal neurons
in vitro
and the dependence on the duration of ClPF action. We also observed
survival of the cells incubated in the media with only ClPF and under
the same conditions but with the addition of Trolox as an antioxidant.
It was found that Trolox demonstrated clear neuroprotective effects at
all concentrations of ClPF tested during the research period. It is
concluded that the negative effect of ClPF on hippocampal neurons
results, to a considerable extent, in the development of oxidative
stress.
CombiMag from OZ Biosciences is a very efficient transfection reagent based on Magnetofection™ technology.
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