HEK transfection with DNA using NeuroMag for chemotaxis analysis

HEK cells were plated on 35-mm dishes (150.000 cells/dish) and transfected 24 h later using Neuromag. 

This paper shows the high efficiency of NeuroMag transfection reagent from OZ Biosciences to transfect HEK cell line. In this study, NeuroMag transfection reagent was used to perform chemotaxis assays 48 h after transfection.

article reference: PLoS One. 2013 Sep 2;8(9):e73426.

Functional Cross Talk between CXCR4 and PDGFR on Glioblastoma Cells Is Essential for Migration.

Sciaccaluga M, D'Alessandro G, Pagani F, Ferrara G, Lopez N, Warr T, Gorello P, Porzia A, Mainiero F, Santoro A, Esposito V, Cantore G, Castigli E, Limatola C.

Abstract

Glioblastoma (GBM) is the most common and aggressive form of brain tumor, characterized by high migratory behavior and infiltration in brain parenchyma which render classic therapeutic approach ineffective. The migratory behaviour of GBM cells could be conditioned by a number of tissue- and glioma-derived cytokines and growth factors. Although the pro-migratory action of CXCL12 on GBM cells in vitro and in vivo is recognized, the molecular mechanisms involved are not clearly identified. In fact the signaling pathways involved in the pro-migratory action of CXCL12 may differ in individual glioblastoma and integrate with those resulting from abnormal expression and activation of growth factor receptors. In this study we investigated whether some of the receptor tyrosine kinases commonly expressed in GBM cells could cooperate with CXCL12/CXCR4 in their migratory behavior. Our results show a functional cross-talk between CXCR4 and PDGFR which appears to be essential for GBM chemotaxis.

NeuroMag is the first dedicated Magnetofection ™ transfection reagent for neurons. It is perfect for primary neurons and can also be used with cell lines and glial cells. The transfection can be performed with neurons from 3 DIV to 21 DIV.Recent data showed that it can be used for other cell types such as cell lines (HEK-293), macrophages or primary cardiomyocytes and cardiac progenitors.