siRNA reverse transfection of MDCK and HCT-116 using Lullaby

In 6-well plate, 37.5 nM siRNA and 10 µL of Lullaby transfection reagent were used to transfect MDCK and HCT-116 cells.

this article demonstrate the high efficiency of Lullaby, siRNA transfection reagent from OZ Biosciences to induce gene silencing in cell lines.

article reference: Sci Signal. 2013 Sep 17;6(293):ra82.

A cancer-associated mutation in atypical protein kinase cι occurs in a substrate-specific recruitment motif.

Linch M, Sanz-Garcia M, Soriano E, Zhang Y, Riou P, Rosse C, Cameron A, Knowles P, Purkiss A, Kjaer S, McDonald NQ, Parker PJ.

 

Abstract

Atypical protein kinase Cι (PKCι) has roles in cell growth, cellular polarity, and migration, and its abundance is frequently increased in cancer. We identified a protein interaction surface containing a dibasic motif (RIPR) that bound a distinct subset of PKCι substrates including lethal giant larvae 2 (LLGL2) and myosin X, but not other substrates such as Par3. Further characterization demonstrated that Arg(471) in this motif was important for binding to LLGL2, whereas Arg(474) was critical for interaction with myosin X, indicating that multiple complexes could be formed through this motif. A somatic mutation of the dibasic motif (R471C) was the most frequent mutation of PKCι in human cancer, and the intact dibasic motif was required for normal polarized epithelial morphogenesis in three-dimensional cysts. Thus, the R471C substitution is a change-of-function mutation acting at this substrate-specific recruitment site to selectively disrupt the polarizing activity of PKCι.

Lullaby® is the ideal siRNA transfection reagent for gene silencing reaching up to 90% gene silencing. It protects siRNA from extracellular degradation and maintains high viability due to its bio-degradable properties.