Primary colon cancer-initiating cells (primary human colorectal cancer or derived metastasis) lentiviral transduction using Magnetofection with ViroMag R/L beads.

To generate stable genetically modified cells, P3 spheroid of primary colon cancer cells (colorectal and metastasis derived) were dissociated and lentivirally transduced using ViroMag R/L beasds.

This paper shows the efficiency of ViroMag R/L from OZ Biosciences to transduce primary colon cancer-initiating cells with lentivirus.

article reference: Nat Commun. 2013 Oct 28;4:2610. 

Wnt secretion is required to maintain high levels of Wnt activity in colon cancer cells.

Voloshanenko O, Erdmann G, Dubash TD, Augustin I, Metzig M, Moffa G, Hundsrucker C, Kerr G, Sandmann T, Anchang B, Demir K, Boehm C, Leible S, Ball CR, Glimm H, Spang R, Boutros M.

Abstract

Aberrant regulation of the Wnt/β-catenin pathway has an important role during the onset and progression of colorectal cancer, with over 90% of cases of sporadic colon cancer featuring mutations in APC or β-catenin. However, it has remained a point of controversy whether these mutations are sufficient to activate the pathway or require additional upstream signals. Here we show that colorectal tumours express elevated levels of Wnt3 and Evi/Wls/GPR177. We found that in colon cancer cells, even in the presence of mutations in APC or β-catenin, downstream signalling remains responsive to Wnt ligands and receptor proximal signalling. Furthermore, we demonstrate that truncated APC proteins bind β-catenin and key components of the destruction complex. These results indicate that cells with mutations in APC or β-catenin depend on Wnt ligands and their secretion for a sufficient level of β-catenin signalling, which potentially opens new avenues for therapeutic interventions by targeting Wnt secretion via Evi/Wls.

ViroMag R/L from OZ Biosciences is a magnetic nanoparticles formulation optimized for increasing any kind of virus infection and transduction both in vitro and in vivo.