Immortal human keratinocyte cell line HaCat transfected with EcoTransfect

HaCat cells were transfected using EcotransFect to generate stable cell lines.

This article illustrates the efficiency of EcoTransfect transfection reagent from OZ Biosciences to stably transfect HaCat cells.

article reference: Mol Oncol. 2014 Jan 14. pii: S1574-7891(14)00003-9.

Human papillomavirus (HPV)-18 E6 oncoprotein interferes with the epithelial cell polarity Par3 protein.

Facciuto F, Bugnon Valdano M, Marziali F, Massimi P, Banks L, Cavatorta AL, Gardiol D.

AbstractHigh-risk human papillomavirus (HPV) infection is the principal risk factor for the development of cervical cancer. The HPV E6 oncoprotein has the ability to target and interfere with several PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins that are involved in the control of cell polarity. This function can be significant for E6 oncogenic activity because a deficiency in cell polarisation is a marker of tumour progression. The establishment and control of polarity in epithelial cells depend on the correct asymmetrical distribution of proteins and lipids at the cell borders and on specialised cell junctions. In this report, we have investigated the effects of HPV E6 protein on the polarity machinery, with a focus on the PDZ partitioning defective 3 (Par3) protein, which is a key component of tight junctions (TJ) and the polarity network. We demonstrate that E6 is able to bind and induce the mislocalisation of Par3 protein in a PDZ-dependent manner without significant reduction in Par3 protein levels. In addition, the high-risk HPV-18 E6 protein promotes a delay in TJ formation when analysed by calcium switch assays. Taken together, the data presented in this study contribute to our understanding of the molecular mechanism by which HPVs induce the loss of cell polarity, with potential implications for the development and progression of HPV-associated tumours.

EcoTransfect from OZ Biosciences is an economical reagent dedicated to the transfection of popular cell lines. It is the perfect solution to quickly analyze the biological activity of your nucleic acids, to perform routine transfection assays at low cost and to accomplish high throughput screening.