Determination of protein level in bronchoalveolar lavage fluid (BALF) of mice using the Bradford Protein Assay Kit from OZ Biosciences was used to measure the

In BALF, protein levels were measured using the Bradford Protein Assay Kit - BPAK from OZ Biosciences.

This article demonstrates the capacity of the Bradford Protein Assay Kit from OZ Biosciences  to efficiently measure the total protein concentration in BALF of mice.

article reference: Beurskens CJ. Intensive Care Medicine Experimental 2014, 2:8 

Mechanical ventilation with heliox in an animal model of acute respiratory distress syndrome

Charlotte J Beurskens, Hamid Aslami, Friso M de Beer, Joris JTH Roelofs, Margreeth B Vroom and Nicole P Juffermans.

Abstract

Background:Heliox has a lower density and higher diffusion capacity compared to oxygen-in-air. We hypothesized that heliox ventilation allows for a reduction in minute volume ventilation and inspiratory pressures needed for adequate gas exchange in an animal model of an acute lung injury.
Methods:After intratracheal instillation of lipopolysaccharide (10 mg/kg), adult rats were randomized to ventilation with either a gas mixture of helium/oxygen (50:50%) or oxygen/air (50:50%). They were mechanically ventilated according to the ARDSnet recommendations with tidal volumes of 6 ml/kg and monitored with a pneumotachometer. Bronchoalveolar lavage fluid was analyzed for markers of lung injury, and embedded lung sections were histologically scored for lung injury.
Results:Heliox limited the increase in driving pressures needed to achieve preset tidal volumes, with a concomitant decrease in loss of compliance. Heliox did neither allow for reduced minute volume ventilation in this model nor improve gas exchange. Also, heliox did not reduce lung injury.
Conclusions:Heliox modestly improved respiratory mechanics but did not improve lung injury in this rat model of acute respiratory distress syndrome.

The Bradford Protein Assay Kit (B-Pak) from OZ Biosciences is a straightforward and rapid procedure for determining the concentration of protein in solution.The B-Pak is based on the binding of Coomassie Brilliant Blue G-250 dye to the proteins and particularly basic and aromatic amino acids residues. The dye exists in three forms: cationic (red), neutral (green) and anionic (blue).