Neural stem/progenitor cells (NSP) transfection with plasmid DNA using NeuroMag (Magnetofection)

Efficient transfection of plasmid into Neural Stem/Progenitor cells (NSP) with NeuroMag magnetic nanoparticles

This paper shows the capacity of NeuroMag from OZ Biosciences to transfect Neural Stem/Progenitor cells (NSP).

article reference: Sci Rep. 2014 May 15;4:4972. doi: 10.1038/srep04972.

Ecto-domain phosphorylation promotes functional recovery from spinal cord injury.

Suehiro K, Nakamura Y, Xu S, Uda Y, Matsumura T, Yamaguchi Y, Okamura H, Yamashita T, Takei Y.

Abstract

Inhibition of Nogo-66 receptor (NgR) can promote recovery following spinal cord injury. The ecto-domain of NgR can be phosphorylated by protein kinase A (PKA), which blocks activation of the receptor. Here, we found that infusion of PKA plus ATP into the damaged spinal cord can promote recovery of locomotor function. While significant elongation of cortical-spinal axons was not detectable even in the rats showing enhanced recovery, neuronal precursor cells were observed in the region where PKA plus ATP were directly applied. NgR1 was expressed in neural stem/progenitor cells (NSPs) derived from the adult spinal cord. Both an NgR1 antagonist NEP1-40 and ecto-domain phosphorylation of NgR1 promote neuronal cell production of the NSPs, in vitro. Thus, inhibition of NgR1 in NSPs can promote neuronal cell production, which could contribute to the enhanced recovery of locomotor function following infusion of PKA and ATP.

NeuroMag is the first dedicated Magnetofection ™ transfection reagent for neurons. It is perfect for primary neurons and can also be used with cell lines and glial cells. The transfection can be performed with neurons from 3 DIV to 21 DIV. It can also be used on ES cell derived motor neurons.