Ovarian cancer cell lines SKOV3 and IGROV1 were transfected with siRNA and co-transfected DNA using DreamFect and DreamFect Gold

SKOV3 and IGROV1 cells grown as monolayers and spheroids were transiently co-transfected with plasmids at a 10:1 ratio using DreamFect Gold transfection reagent. siRNA were efficiently delivered into SKOV3 with DreamFect.

This article demonstrates the high efficiency of DreamFect Gold from OZ Biosciences for DNA transfection into ovarian cancer cell lines and the capacity of DreamFect to silence gene expression in SKOV3.

article reference: Oncogene. 2014 Jun 23. doi: 10.1038/onc.2014.178. 

β-Catenin-regulated ALDH1A1 is a target in ovarian cancer spheroids.

Condello S, Morgan CA, Nagdas S, Cao L, Turek J, Hurley TD, Matei D.

Abstract

Cancer cells form three-dimensional (3D) multicellular aggregates (or spheroids) under non-adherent culture conditions. In ovarian cancer (OC), spheroids serve as a vehicle for cancer cell dissemination in the peritoneal cavity, protecting cells from environmental stress-induced anoikis. To identify new targetable molecules in OC spheroids, we investigated gene expression profiles and networks upregulated in 3D vs traditional monolayer culture conditions. We identified ALDH1A1, a cancer stem cell marker as being overexpressed in OC spheroids and directly connected to key elements of the β-catenin pathway. β-Catenin function and ALDH1A1 expression were increased in OC spheroids vs monolayers and in successive spheroid generations, suggesting that 3D aggregates are enriched in cells with stem cell characteristics. β-Catenin knockdown decreased ALDH1A1 expression levels and β-catenin co-immunoprecipitated with the ALDH1A1 promoter, suggesting that ALDH1A1 is a direct β-catenin target. Both short interfering RNA-mediated β-catenin knockdown and A37 ((ethyl-2-((4-oxo-3-(3-(pryrrolidin-1-yl)propyl)-3,4-dihydrobenzo [4,5]thioeno [3,2-d]pyrimidin-2-yl)thio)acetate)), a novel ALDH1A1 small-molecule enzymatic inhibitor described here for the first time, disrupted OC spheroid formation and cell viability (P<0.001). β-Catenin knockdown blocked tumor growth and peritoneal metastasis in an OC xenograft model. These data strongly support the role of β-catenin-regulated ALDH1A1 in the maintenance of OC spheroids and propose new ALDH1A1 inhibitors targeting this cell population.Oncogene advance online publication, 23 June 2014; doi:10.1038/onc.2014.178.

DreamFect™Gold allows transfecting all types of nucleic acids with a very high efficiency. Due to its formulation, DreamFect™Gold delivers a large quantity of nucleic acids leading to higher protein expression compared to other transfection reagents. It is fully biodegradable and does not interfere with cellular mechanisms.

DreamFect from OZ Biosciences allows transfecting all types of nucleic acids with a very high efficiency; it is fully biodegradable and does not interfere with cellular mechanisms.