Co-transfection of plasmid DNA into HEK293 (HEK-293) using DreamFect Gold

Transfection of Human embryonic kidney (HEK) 293 cells were carried out in T25 flasks at 60-80% confluency using 10 µL DreamFect Gold transfection reagent and 2.5 µg total plasmid DNA. 

This article demonstrates the high efficiency of DreamFect Gold from OZ Biosciences for co-transfection or plasmid DNA in cell lines.

article reference: Peptides. 2014 Jul 23. pii: S0196-9781(14)00214-9. doi: 10.1016/j.peptides.2014.07.014.

Cloning, constitutive activity and expression profiling of two receptors related to relaxin receptors in Drosophila melanogaster.

Van Hiel MB, Vandersmissen HP, Proost P, Vanden Broeck J.

AbstractLeucine-rich repeat containing G protein-coupled receptors (LGRs) comprise a cluster of transmembrane proteins, characterized by the presence of a large N-terminal extracellular domain. This receptor group can be classified into three subtypes. Belonging to the subtype C LGRs are the mammalian relaxin receptors LGR7 (RXFP1) and LGR8 (RXFP2), which mediate important reproductive and other processes. We identified two related receptors in the genome of the fruit fly and cloned their open reading frames into an expression vector. Interestingly, dLGR3 demonstrated constitutive activity at very low doses of transfected plasmid, whereas dLGR4 did not show any basal activity. Both receptors exhibited a similar expression pattern during development, with relatively high transcript levels during the first larval stage. In addition, both receptors displayed higher expression in male adult flies as compared to female flies. Analysis of the tissue distribution of both receptor transcripts revealed a high expression of dLGR3 in the female fat body, while the expression of dLGR4 peaked in the midgut of both the wandering and adult stage.

DreamFect™Gold allows transfecting all types of nucleic acids with a very high efficiency. Due to its formulation, DreamFect™Gold delivers a large quantity of nucleic acids leading to higher protein expression compared to other transfection reagents. It is fully biodegradable and does not interfere with cellular mechanisms.