Bacterial protein concentration determined using BPAK - Bradford Protein Assay Kit from OZ Biosciences

After heat-inactivation (80°C x 2h), protein concentration of mycobacteria was quantified with the BPAK - Bradford Protein Assay Kit.

This article demonstrate the capacity of the Bradford Protein Assay Kit from OZ Biosciences  to efficiently measure the total protein concentration of bacteria.

article reference: Immunology. 2014 Oct 16. doi: 10.1111/imm.12411.

CD11c+CD103+cells of Mycobacterium tuberculosis-infected C57BL/6 but not of BALB/c mice induce a high frequency of IFN-γ- or IL-17-producing CD4+cells.

Sérgio CA, Bertolini TB, Gembre AF, de Queiroz Prado R, Bonato VL.
AbstractThe magnitude of the cellular adaptive immune response is critical for the control of Mycobacterium tuberculosis-infection in the chronic phase. In addition, the genetic background is equally important for resistance or susceptibility to tuberculosis. In this study, we addressed whether lung populations of dendritic cells, obtained from genetically different hosts, would play a role in the magnitude and function of CD4⁺ populations generated after M. tuberculosis infection. Thirty days post-infection, C57BL/6 mice, which generate a stronger IFN-γ-mediated immune response than BALB/c mice, exhibited a higher number and frequency of lung CD11c⁺ CD11b^- CD103⁺ cells compared to BALB/c mice, which exhibited a high frequency of lung CD11c⁺ CD11b⁺ CD103^- cells. CD11c⁺ CD11b^- CD103⁺ cells, purified from lungs of infected C57BL/6 mice, but not from infected BALB/c mice, induced a higher frequency of IFN-γ- or IL-17-producing CD4⁺ cells. Moreover, CD4⁺ cells also arrive at the lung of C57BL/6 mice faster than in BALB/c mice. This pattern of immune response seems to be associated with higher gene expression for CCL4, CCL19, CCL20 and CCR5 in the lung of infected C57BL/6 mice compared to infected BALB/c mice. The results described here show that the magnitude of IFN-γ- or IL-17-producing CD4⁺ cells is dependent on CD11c⁺ CD11b^- CD103⁺ cells, and this pattern of immune response is directly associated with the host genetic background. Therefore, differences in the genetic background contribute to the identification of immunological biomarkers that can be used to design human assays to predict progression of M. tuberculosis-infection. This article is protected by copyright. All rights reserved.

The Bradford Protein Assay Kit (B-Pak) from OZ Biosciences is a straightforward and rapid procedure for determining the concentration of protein in solution.The B-Pak is based on the binding of Coomassie Brilliant Blue G-250 dye to the proteins and particularly basic and aromatic amino acids residues. The dye exists in three forms: cationic (red), neutral (green) and anionic (blue).