DNA transfection of sf9, ovarian insect cells from spodoptera fugiperda with Flyfectin

One microgram of purified DNA was transfected in sf9 cells with Flyfectin for 96 H.

This paper shows the efficiency of Flyfectin from OZ Biosciences to transfect sf9 insect cells.

article reference: Gene Ther. 2014 Oct 23. doi: 10.1038/gt.2014.89. 

Delivery of glutamine synthetase gene by baculovirus vectors: a proof of concept for the treatment of acute hyperammonemia.

Torres-Vega MA, Vargas-Jerónimo RY, Montiel-Martínez AG, Muñoz-Fuentes RM, Zamorano-Carrillo A, Pastor AR, Palomares LA.

Abstract
Hyperammonemia, a condition present in patients with urea cycle disorders (UCDs) or liver diseases, can cause neuropsychiatric complications, which in the worst cases result in brain damage, coma or death. Diverse treatments exist for the treatment of hyperammonemia, but they have limited efficacy, adverse effects and elevated cost. Gene therapy is a promising alternative that is explored here. A baculovirus, termed Bac-GS, containing the glutamine synthetase (GS) gene was constructed for the in vitro and in vivo treatment of hyperammonemia. Transduction of MA104 epithelial or L6 myoblast/myotubes cells with Bac-GS resulted in a high expression of the GS gene, an increase in GS concentration, and a reduction of almost half of exogenously added ammonia. When Bac-GS was tested in an acute hyperammonemia rat model by intramuscularly injecting the rear legs, the concentration of ammonia in blood decreased 351 μM, in comparison with controls. A high GS concentration was detected in gastrocnemius muscles from the rats transduced with Bac-GS. These results show that gene delivery

FlyFectin™ from OZ Biosciences is a powerful reagent specifically designed to obtain highly efficient and reproducible transfection of insect cells. It is adapted to the delivery of all types of nucleic acids. FlyFectin™ can be used for many applications including for the production of recombinant protein using Baculovirus expression system