Magnetofectamine Kit for BEAS-2B efficient transfection with plasmid DNA and siRNA

30000 BEAS-2B were seeded in 96-well plate for 24H before transfection with 0.2 µg plasmid using the Magnetofectamine kit.

This paper demonstrated the high efficiency of Magnetofectamine kit from OZ Biosciences for DNA delivery into BEAS-2B.

article reference: Oncotarget. 2014 Jun 15;5(11):3862-70.

Sequential activation of Elk-1/Egr-1/GADD45α by arsenic.

Shi Q¹, Sutariya V, Bishayee A, Bhatia D².

Abstract

Long-term exposure to arsenic, an environmental contaminant, leads to increased risks of cancers. In the present study, we investigated the sequential regulation of Elk-1 and Egr-1 on As3+-induced GADD45α, an effector of G2/M checkpoint. We found that As3+ transcriptionally induced both Elk-1 and Egr-1, and NF-κB binding site was necessary for As3+-induced Egr-1 promoter activity. However, specific inhibition of JNK, ERK, and Elk-1 inhibited Egr-1 induction. Furthermore, silencing of Egr-1 downregulated As3+-induced expression of GADD45α and ChIP assay confirmed the direct binding of Egr-1 to GADD45α promoter. Taken together, our data indicated that the increase of GADD45α in response to As3+ was mediated sequentially by Elk-1 and Egr-1.

Magnetofectamine from OZ Biosciences is a very efficient transfection reagent based on Magnetofection™ technology.