D-Luciferin from OZ Biosciences to monitor Luciferase activity of B16 melanoma cells in vivo

B16-luciferase melanoma cells were injected into tail vein. 3 mg/mouse of D-Luciferin from OZ Biosciences was injected intraperitoneally to monitor luciferase expression by in vivo imaging.

This article demonstrates the capacity of the D-Luciferin from OZ Biosciences  to efficiently monitor luciferase expression in vivo.

article reference: Nat Commun. 2014 Oct 30;5:5108.

SHP-1-mediated inhibitory signals promote responsiveness and anti-tumour functions of natural killer cells.

Viant C, Fenis A, Chicanne G, Payrastre B, Ugolini S, Vivier E.

abstract 

Natural killer (NK) cells are cytotoxic innate lymphoid cells that are involved in immune defense. NK cell reactivity is controlled in part by MHC class I recognition by inhibitory receptors, but the underlying molecular mechanisms remain undefined. Using a mouse model of conditional deletion in NK cells, we show here that the protein tyrosine phosphatase SHP-1 is essential for the inhibitory function of NK cell MHC class I receptors. In the absence of SHP-1, NK cells are hyporesponsive to tumour cells in vitro and their early Ca(2+) signals are compromised. Mice without SHP-1 in NK cells are unable to reject MHC class I-deficient transplants and to control tumours in vivo. Thus, the inhibitory activity of SHP-1 is needed for setting the threshold of NK cell reactivity.  

D-Luciferin K+ and Na+ salts are dedicated to in vitro and in vivo bioluminescent assays. The quality and purity of the D-Luciferin are essential to obtain good and reproducible results. OZ Biosciences is offering high quality of Endotoxin-Free D-Luciferin K+ and Na+ salts.