Use of HYPE-5 to produce viral E proteins which can confer in vivo protection against ZIKA virus

Structural Basis of Zika Virus-Specific Antibody Protection

Cell July 2016
Haiyan Zhao et al.

Abstract 

Zika virus (ZIKV) infection during pregnancy has emerged as a global public health problem because of its ability to cause severe congenital disease. Here, we developed six mouse monoclonal antibodies (mAbs) against ZIKV including four (ZV-48, ZV-54, ZV-64, and ZV-67) that were ZIKV specific and neutralized infection of African, Asian, and American strains to varying degrees. X-ray crystallographic and competition binding analyses of Fab fragments and scFvs defined three spatially distinct epitopes in DIII of the envelope protein corresponding to the lateral ridge (ZV-54 and ZV-67), C-C' loop (ZV-48 and ZV-64), and ABDE sheet (ZV-2) regions. In vivo passive transfer studies revealed protective activity of DIII-lateral ridge specific neutralizing mAbs in a mouse model of ZIKV infection. Our results suggest that DIII is targeted by multiple type-specific antibodies with distinct neutralizing activity, which provides a path for developing prophylactic antibodies for use in pregnancy or designing epitope-specific vaccines against ZIKV.

HYPE-5 Transfection Kit is dedicated to achieve High Yield Protein Expression in mammalian cells. This Kit has been designed for maximum recombinant protein expression in HEK293 and CHO cells growing in suspension. It is composed of the HYPE-5 transfection reagent (a Lipofection reagent) and HYPE-Blast reagent (improves protein production in CHO cells). The HYPE-5™ transfection kit is ideal for bioreactor, spinner or flasks.