Protein delivery in Adipocyte Stem cells using Pro-DeliverIN

25 µL of functional cytosolic fractions were delivered to 250000 Adipocyte Stem Cells seeded on a well of a 6-well okate using Pro-DeliverIN reagent.

This paper shows the high efficiency of Pro-DeliverIN from OZ Biosciences to deliver proteins into Stem Cells. Adipocyte Stem Cells were used in this paper.

article reference: Diabetes. 2013 Feb 19.

Metabolic rescue of obese adipose-derived stems cells by Lin28/Let7 pathway.

 Pérez LM, Bernal A, San Martín N, Lorenzo M, Fernández-Veledo S, Gálvez BG.

Abstract

Adipose-derived stem cells (ASCs) are promising candidates for autologous cell-based regeneration therapies by virtue of their multilineage differentiation potential and immunogenicity, however relatively little is known about their role in adipose tissue physiology and dysfunction. Here we evaluated whether ASCs isolated from non-obese and obese tissue differed in their metabolic characteristics and differentiation potential. During differentiation to mature adipocytes, both mouse and human ASCs derived from non-obese tissues increased their insulin sensitivity and inhibition of lipolysis while obese-derived ASCs were insulin-resistant, showing impaired insulin-stimulated glucose uptake and resistance to the antilipolytic effect of insulin. Furthermore, obese-derived ASCs showed enhanced release of proinflammatory cytokines and impaired production of adiponectin. Interestingly, the delivery of cytosol from control ASCs into obese-derived ASCs using a lipid-based protein-capture methodology restored insulin sensitivity on glucose and lipid metabolism and reversed the proinflammatory cytokine profile, in part due to the restoration of Lin28 protein levels. In conclusion, glucose and lipid metabolism as well as maturation of ASCs are truncated in an obese environment. The reversal of the altered pathways in obese cells by delivery of normal subcellular fractions offers a potential new tool for cell therapy.

Pro-DeliverIN™ is an innovative reagent allowing intracellular delivery of biologically active proteins. This lipid-based formulation is the first serum compatible reagent to deliver functional proteins into living cells. Consequently, it is the only reagent which allows livering protein in vitro and in vivo.