COS7 transfection using PolyMag Neo

Plasmid DNA were transiently transfected into COS7 (COZ-7) cell line using Magnetofection technology with PolyMag Neo transfection reagent.

This paper shows the high efficiency of PolyMag Neo transfection reagent from OZ Biosciences to transfect DNA into cell line.

paper reference: J Clin Microbiol. 2013 May 8.

Highly sensitive detection of hepatitis B virus surface antigen using a semi-automated immune complex transfer chemiluminescent enzyme immunoassay.
Takeda K, Maruki M, Yamagaito T, Muramatsu M, Sakai Y, Tobimatsu H, Kobayashi H, Mizuno Y, Hamaguchi Y.

Abstract

The performance of hepatitis B surface antigen (HBsAg) screening assays is continuously improved to reduce the risk of transfusion-associated hepatitis B. In this study, a semi-automated immune complex transfer chemiluminescence enzyme immunoassay (ICT-CLEIA) for the detection of HBsAg, which is as sensitive as hepatitis B virus (HBV)-DNA polymerase chain reaction (PCR), was developed; the ICT-CLEIA assay performance was compared with the performance of the Architect HBsAg QT assay and HBV-DNA PCR. The specificities in the initial assay and after retesting were 99.50% (1,988/1,998) and 99.95% (1,997/1,998), respectively. The analytical detection limit was determined to be 0.2 mIU/mL using the 2nd International WHO HBsAg standard, and the cutoff value (0.5 mIU/mL) of the ICT-CLEIA assay was 8.0 standard deviations (SD) above the mean of the HBsAg-negative specimens. The ICT-CLEIA assay could detect HBsAg even in the presence of anti-HBs antibodies and demonstrated a 23.6-day shorter window periods using commercially available HBsAg seroconversion panels when compared to the Architect HBsAg QT assay. Furthermore, the monitoring of the viral kinetics by the ICT-CLEA assay and the HBV-DNA PCR produced very similarly shaped curves during both the HBsAg seroconversion and reverse seroconversion periods. Therefore, the ICT-CLEIA assay may be useful not only for an earlier detection of HBV reactivation but also for the monitoring of hepatitis B patients.

The universal and powerful PolyMag Neo formulation allows you to achieve very high transfection efficiency. It is based on Magnetofection™ transfection technology. PolyMag Neo reagent has been optimized to be used with all types of nucleic acids.