Gene Silencing in ovarian cancer cell line SKOV3 [SK-VO-3] [SKOV-3] using DreamFect transfection reagent

Transient transfection of siRNA targetting beta-catenin was performed using DreamFect reagent in ovarian cancer cell line.

This article demonstrate the versatility of Dreamfect transfection reagent from OZ Biosciences to efficiently deliver siRNA into SKOV3 cell line.

article reference: FASEB J. 2013 May 2.

Tissue transglutaminase regulates β-catenin signaling through a c-Src-dependent mechanism.

Condello S, Cao L, Matei D.

Abstract

Tissue transglutaminase (TG2) is a multifunctional enzyme involved in protein cross-linking and cell adhesion to fibronectin (FN). In cancer, TG2 induces an epithelial to mesenchymal transition, contributing to metastasis. Because cadherins bind β-catenin at cell-cell junctions, disruption of adherens junctions destabilizes cadherin-catenin complexes. The goal of the present study was to analyze whether and how TG2 interacts with and regulates β-catenin signaling in ovarian cancer (OC) cells. We observed a significant correlation between TG2 and β-catenin expression levels in OC cells and tumors. TG2 augmented Wnt/β-catenin signaling, as evidenced by enhanced β-catenin transcriptional activity, inducing transcription of target genes cyclin D1 and c-Myc. By promoting integrin-mediated cell adhesion to FN, TG2 physically associates with and recruits c-Src, which in turn phosphorylates β-catenin at Tyr⁶⁵⁴, releasing it from E-cadherin and rendering it available for transcriptional regulation. By interacting with FN and enhancing β-catenin signaling, complexed TG2 stimulates OC cell proliferation. In summary, our data demonstrate that TG2 regulates β-catenin expression and function in OC cells and define the c-Src-dependent mechanism through which this occurs.-Condello, S., Cao, L., Matei, D. Tissue transglutaminase regulates β-catenin signaling through a c-Src-dependent mechanism.

DreamFect from OZ Biosciences allows transfecting all types of nucleic acids with a very high efficiency; it is fully biodegradable and does not interfere with cellular mechanisms.