Primary cells and cell lines transfection in 3D dimensions using 3D-Fect and 3D-FectIN - 3D transfection reagents

3D-Fect and 3D-FectIN, two reagents dedicated to transfection in 3D - three dimensions - were successfully used to transfect primary and cell lines onto 3D-scaffolds and hydrogels (gels) respectively with DNA and siRNA.

Human cervical carcinoma (HeLa), HeLa-GFP, human embryonic kidney (HEK-293), African green monkey fibroblast (COS-7), MDCK, mouse fibroblasts (NIH-3T3), human glial (oligodendrocytic) hybrid (CLU-301) cell lines, murine macrophage cell line (Raw 264.7), Human microvascular endothelial cell line 1 (HMEC-1), Mouse embryonic fibroblasts (MEF), Human adipocyte stromal cells, Human mesenchymal stem cells (hMSC), Rabbit primary chondrocytes, mouse Neural stem cells were transfected in 3D (three dimensions) onto 3D-scaffolds or into hydrogels using 3D-Fect and 3D-FectIN transfection reagents from OZ Biosciences.

article reference: Ther Deliv. 2013 Jun;4(6):673-85.

3D-fection: cell transfection within 3D scaffolds and hydrogels.

Sapet C, Formosa C, Sicard F, Bertosio E, Zelphati O, Laurent N.

Abstract

Background: 3D matrices are widely used as cell growth supports in basic research, regenerative medicine or cell-based drug assays. In order to genetically manipulate cells cultured within 3D matrices, two novel non-viral transfection reagents allowing preparation of matrices for in situ cell transfection were evaluated. Results: Two lipidic formulations, 3D-Fect™ and 3D-FectIN™, were assessed for their ability to transfect cells cultured within 3D solid scaffolds and 3D hydrogels, respectively. These reagents showed good compatibility with the most widespread types of matrices and enabled transfection of a wide range of mammalian cells of various origins. Classical cell lines, primary cells and stem cells were thus genetically modified while colonizing their growth support. Importantly, this in situ strategy alleviated the need to manipulate cells before seeding them. Conclusion: Results presented here demonstrated that 3D-Fect and 3D-FectIN reagents for 3D transfection are totally compatible with cells and do not impair matrix properties. 3D-Fect and 3D-FectIN, therefore, provide valuable tools for achieving localized and sustained transgene expression and should find versatile applications in fundamental research, regenerative medicine and cell-based drug assays.

3D matrices not only add a third dimension to cells’ environment, they also allow creating significant differences in cellular characteristics and behavior.  

3D-Fect™ is the newest reagent specifically developed to directly transfect cells cultured in 3D scaffold. 3D-Fect™ is suitable for all kind of scaffolds and cells.

3D-FectIN™ is the newest reagent specifically developed to directly transfect cells cultured in 3D hydrogel. 3D-FectIN™ is suitable for all kind of hydrogels and cells.