In vivo monitoring of luciferase activity with Luciferin from OZ Biosciences

Intraperitoneal injection of 40 mg/kg luciferin from OZ Biosciences were used to monitor luciferase expression in head and neck squamous cell carcinoma metastasis.

This article demonstrate the capacity of the Luciferin from OZ Biosciences  to efficiently monitor luciferase expression in vivo.

article reference: Head Neck. 2013 Jun 1.

Enhancement of head and neck squamous cell carcinoma proliferation, invasion, and metastasis by tumor-associated fibroblasts in preclinical models.

Wheeler SE, Shi H, Lin F, Dasari S, Bednash J, Thorne S, Watkins S, Joshi R, Thomas SM.

Abstract

BACKGROUND: Head and neck squamous cell carcinoma (HNSCC) has had little improvement in mortality rates in decades. A clearer understanding of the HNSCC tumor microenvironment will aid in finding more effective targeted therapies for this disease. Tumor-associated fibroblasts (TAFs) are the largest stromal cellular components of the tumor microenvironment in HNSCC.
METHODS: We isolated TAFs from clinical HNSCC cases and propagated in vitro. The effects of TAF-secreted paracrine factors on in vitro HNSCC migration, invasion, and proliferation was assessed. The effect of TAFs on HNSCC growth and metastases was determined in an orthotopic floor-of-the-mouth tumor model.
RESULTS: TAF-conditioned media increased HNSCC cell migration, invasion, and proliferation. TAFs increased HNSCC tumor growth and metastases in vivo.
CONCLUSION: TAFs play a major role in increasing tumor growth and metastasis in HNSCC. Targeting the tumor stroma may be important to reduce the rate of HNSCC metastasis. © 2013 Wiley Periodicals, Inc. Head Neck, 2013.

D-Luciferin K⁺ and Na⁺ salts are dedicated to in vitro and in vivo bioluminescent assays. The quality and purity of the D-Luciferin are essential to obtain good and reproducible results. OZ Biosciences is offering high quality of Endotoxin-Free D-Luciferin K⁺ and Na⁺ salts.