Determination of protein concentration in Salivary gland extracts (SGE) of aedes albopictus was performed using Bradford Protein Assay Kit (B-Pak) from OZ Biosciences.
This article demonstrate the capacity of the Bradford Protein Assay Kit from
OZ Biosciences to efficiently measure the protein concentration in SGE from
aedes albopictus.
First screening of Aedes albopictus immunogenic salivary proteins.
Doucoure S,
Cornelie S,
Patramool S,
Mouchet F,
Demettre E,
Seveno M,
Dehecq JS,
Rutee H,
Herve JP,
Favier F,
Missé D,
Gasque P,
Remoue F.
Abstract
Study of the human antibody (Ab) response to
Aedes salivary proteins can provide new biomarkers to evaluate human exposure to vector bites. The identification of genus- and/or species-specific
proteins is necessary to improve the accuracy of biomarkers. We analysed
Aedes albopictus immunogenic salivary proteins by 2D immunoproteomic technology and compared the profiles according to human individual exposure to
Ae. albopictus or
Ae. aegypti bites. Strong antigenicity to
Ae. albopictus salivary proteins was detected in all individuals whatever the nature of
Aedes exposure. Amongst these antigenic
proteins,
68% are involved in blood feeding, including D7 protein family,
adenosine deaminase, serpin and apyrase. This study provides an insight
into the repertoire of
Ae. albopictus immunogenic salivary proteins for the
first time.
The Bradford Protein Assay Kit (B-Pak) from OZ Biosciences is a straightforward and rapid procedure for determining the concentration of protein in solution.
The B-Pak is based on the binding of Coomassie Brilliant Blue G-250 dye to the proteins and particularly basic and aromatic amino acids residues. The dye exists in three forms: cationic (red), neutral (green) and anionic (blue).
No comments:
Post a Comment