Assay Kit: Total protein levels measurement in BALF supernatants of Mice using the Bradford Protein Assay Kit from OZ Biosciences

Total protein level determination in Bronchoalveolar lavage fluid (BALF) of mice right lung using Bradford Protein Assay Kit (B-Pak) from OZ Biosciences.

This article demonstrate the capacity of the Bradford Protein Assay Kit from OZ Biosciences  to efficiently measure the total protein concentration in BALF of mice lung.

article reference: Transfusion. 2013 Aug 27. doi: 10.1111/trf.12394.

Methylprednisolone fails to attenuate lung injury in a mouse model of transfusion related acute lung injury.

Müller MC, Tuinman PR, van der Sluijs KF, Boon L, Roelofs JJ, Vroom MB, Juffermans NP.

Abstract
BACKGROUND: Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-related morbidity and mortality. Anecdotally, TRALI patients have been treated with corticosteroids. However, evidence for its therapeutic rationale in TRALI is lacking. We determined the effects of corticosteroids on lung injury in a "two-hit" mouse model of antibody-mediated TRALI.
STUDY DESIGN AND METHODS: BALB/c mice were primed with lipopolysaccharide, after which TRALI was induced by injecting major histocompatibility complex (MHC)-I antibody against H2Kd . Mice infused with phosphate-buffered saline served as controls. Simultaneously, one group of TRALI mice was infused with methylprednisolone (MPS; 2 mg/kg). Mice were supported by mechanical ventilation for 2 hours, after which bronchoalveolar lavage fluid (BALF) and lung homogenate were obtained. Statistics were obtained by one-way analysis of variance or Kruskal-Wallis.
RESULTS: Injection of MHC-I antibodies resulted in TRALI, indicated by pulmonary edema and increased BALF levels of protein and the proinflammatory mediators macrophage inflammatory protein-2, keratinocyte-derived chemokine, and interleukin (IL)-6. Administration of MPS did not affect the amount of edema nor pulmonary protein and chemokine levels. MPS reduced systemic inflammatory reaction as well as IL-6 levels in the BALF.
CONCLUSION: In a two-hit model of antibody-mediated TRALI, MPS attenuated the IL-6 host response, but failed to prevent the development of lung injury. 

The Bradford Protein Assay Kit (B-Pak) from OZ Biosciences is a straightforward and rapid procedure for determining the concentration of protein in solution. The B-Pak is based on the binding of Coomassie Brilliant Blue G-250 dye to the proteins and particularly basic and aromatic amino acids residues. The dye exists in three forms: cationic (red), neutral (green) and anionic (blue).