Primary amnion epithelial cells at,50% confluence were transfected using SilenceMag reagent (Oz Biosciences, Marseille, France). Transfection was performed using 2000nM siRNA for 72H.
This article highlights the capacity of Magnetofection technology to efficiently silence gene epxression into primary amnion epithelial cells with siRNA using SilenceMag from OZ Biosciences.article reference: Reprod Fertil Dev. 2013 Aug 19.
FOXM1 is lower in human fetal membranes after spontaneous preterm labour and delivery.
AbstractSpontaneous preterm birth is usually associated with infection, inflammation or both. Forkhead box (FOX) M1 (FOXM1), a member of the FOX family of transcription factors, has been associated with inflammation. The aim of this study was to determine whether FOXM1 regulates the expression and release of pro-labour mediators in human gestational tissues. FOXM1 mRNA and protein expression were determined in fetal membranes from women at (1) preterm no labour: Caesarean section with no labour and (2) preterm labour: after spontaneous labour and delivery. Primary amnion cells were utilised to investigate the effect of small interfering RNA (siRNA)-mediated gene silencing of FOXM1 on pro-labour mediators. Spontaneous preterm labour decreased FOXM1 gene and nuclear protein expression. FOXM1 silencing in primary amnion cells increased interleukin (IL)-1b-induced pro-inflammatory cytokines (IL-6 and IL-8 mRNA expression and secretion), cyclooxygenase (COX)-2 expression and subsequent prostaglandin (PG)E2 and PGF2a release as well as gene expression and secretion of the matrix-degrading enzyme matrix metalloproteinase 9 (MMP-9). In conclusion, spontaneous preterm labour is associated with decreasedFOXM1expression in fetal membranes.
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