Transfection of Cardiac Progenitors and Cardiomyocytes with NeuroMag

Cardiac Progenitor cells and Cardiomyocytes were transfected using 0.05 or 0.1 mg of plasmid complexed with 0.2 or 0.35 µl NeuroMag for 15 minutes in serum-free growth media

This paper shows the high efficiency of NeuroMag transfection reagent from OZ Biosciences to transfect Cardiac Progenitors and Cardiomyocytes. Moreover, NeuroMag magnetic transfection vector was used for its non-interference with proliferation and low cytotoxicity, as
observed during transfection of oligodendrocyte progenitor.

article reference: PLoS One. 2013 Jul 31;8(7):e69812.

Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes.

Subramanian M, Lim J, Dobson J.

Abstract

Magnetic nanoparticle-based gene transfection has been shown to be an effective, non-viral technique for delivery of both plasmid DNA and siRNA into cells in culture. It has several advantages over other non-viral delivery techniques, such as short transfection times and high cell viability. These advantages have been demonstrated in a number of primary cells and cell lines. Here we report that oscillating magnet array-based nanomagnetic transfection significantly improves transfection efficiency in both human prenatal cardiac progenitor cells and adult cardiomyocytes when compared to static magnetofection, cationic lipid reagents and electroporation, while maintaining high cell viability. In addition, transfection of adult cardiomyocytes was improved further by seeding the cells onto Collagen I-coated plates, with transfection efficiencies of up to 49% compared to 24% with lipid reagents and 19% with electroporation. These results demonstrate that oscillating nanomagnetic transfection far outperforms other non-viral transfection techniques in these important cells.

NeuroMag is the first dedicated Magnetofection ™ transfection reagent for neurons. It is perfect for primary neurons and can also be used with cell lines and glial cells. The transfection can be performed with neurons from 3 DIV to 21 DIV.Recent data showed that it can be used for other cell types such as cell lines (HEK-293) or primary cardiomyocytes and cardiac progenitors.