DreamFect Gold was chosen among 6 transfection reagent to transfect PMEF and PFF (primary Mouse Embryonic Fibroblasts and primary porcine fibroblasts). Its association with Magnetofection allowed transfecting up to 60 % of these primary cells.
This article demonstrates the high efficiency of DreamFect Gold from OZ Biosciences for DNA transfection. Moreover its association with Magnetofection allow transfection of Primary cells.article reference: Pharm Res. 2014 Jul 18.
Nanomagnetic Activation as a Way to Control the Efficacy of Nucleic Acid Delivery.
Grześkowiak BF, Sánchez-Antequera Y, Hammerschmid E, Döblinger M, Eberbeck D, Woźniak A, Słomski R, Plank C, Mykhaylyk O.
Abstract
PURPOSE:To explore the potential of magnetofection in delivering pDNA to primary mouse embryonic fibroblasts (PMEFs) and porcine fetal fibroblasts (PFFs) and investigate an effect of magnetic cell labeling on transfection efficacy.
METHODS: The formulation and a dose of the magnetic vector were optimized. The efficacy of the procedure was quantified by vector internalization, transgene expression and cell iron loading upon specific labeling with Ab-conjugated magnetic beads or non-specific labeling with MNPs.
RESULTS: Up to sixty percent of PMEF and PFF cells were transfected at low pDNA doses of 4-16 pg pDNA/cell. Specific labeling of the PMEFs with MNPs, resulted in a 3- and 2-fold increase in pDNA internalization upon magnetofection and lipofection, respectively, that yielded a 2-4-fold increase in percent of transgene-expressing cells. Non-specific cell labeling had no effect on the efficacy of the reporter expression, despite the acquisition of similar magnetic moments per cell. In PFFs, specific magnetic labeling of the cell surface receptors inhibited internalization and transfection efficacy.
CONCLUSIONS: Magnetic labeling of cell-surface receptors combined with the application of an inhomogenous magnetic field (nanomagnetic activation) can affect the receptor-mediated internalization of delivery vectors and be used to control nucleic acid delivery to cells.
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