Primary cultures of mice midbrain dopaminergic neurons treated with AraC were efficiently transfected using NeuroMag (Magnetofection)

Primary cultures of dopaminergic neurons derived from E15 mice were transfected with NeuroMag transfection reagent from OZ Biosciences.

This paper shows the high efficiency of NeuroMag from OZ Biosciences to transfect any kind of primary neurons. This is the first paper demonstrated the use of NeuroMag for transfection of Dopaminergic Neurons.

article reference: Neuron. 2014 Jul 16;83(2):404-16. doi: 10.1016/j.neuron.2014.05.043.

Amphetamine Modulates Excitatory Neurotransmission through Endocytosis of the Glutamate Transporter EAAT3 in Dopamine Neurons.

Underhill SM, Wheeler DS, Li M, Watts SD, Ingram SL, Amara SG.

Abstract
Amphetamines modify the brain and alter behavior through mechanisms generally attributed to their ability to regulate extracellular dopamine concentrations. However, the actions of amphetamine are also linked to adaptations in glutamatergic signaling. We report here that when amphetamine enters dopamine neurons through the dopamine transporter, it stimulates endocytosis of an excitatory amino acid transporter, EAAT3, in dopamine neurons. Consistent with this decrease in surface EAAT3, amphetamine potentiates excitatory synaptic responses in dopamine neurons. We also show that the process of internalization is dynamin- and Rho-mediated and requires a unique sequence in the cytosolic C terminus of EAAT3. Introduction of a peptide based on this motif into dopamine neurons blocks the effects of amphetamine on EAAT3 internalization and its action on excitatory responses. These data indicate that the internalization of EAAT3 triggered by amphetamine increases glutamatergic signaling and thus contributes to the effects of amphetamine on neurotransmission.

NeuroMag is the first dedicated Magnetofection™ transfection reagent for neurons. It is perfect for primary neurons and can also be used with cell lines and glial cells. The transfection can be performed with neurons from 3 DIV to 21 DIV. It can also be used on Neural Stem cells as well as cells derived from NSC and Dopaminergic Neurons.