DNA transfection in LNCap (Lymph node cancer prostate cells) using Magnetofection and LipoMag Kit.

LNCap cells were transfected with 1 µg DNA in Lab-Tek slides or 2 µg DNA per well in 12-well plates using LipoMag Kit (association of DreamFect Gold and CombiMag).

This article demonstrates the high efficiency of the association of Magnetofection (CombiMag) to Lipofection (DreamFect Gold) in a simple versatile Kit LipoMag - OZ Biosciences, for DNA transfection into LNCap cells cultivated on several dish formats.

article reference: Eur J Cancer. 2014 Oct 9. pii: S0959-8049(14)00959-9.

Secretogranin II is overexpressed in advanced prostate cancer and promotes the neuroendocrine differentiation of prostate cancer cells.

Courel M, El Yamani FZ, Alexandre D, El Fatemi H, Delestre C, Montero-Hadjadje M, Tazi F, Amarti A, Magoul R, Chartrel N, Anouar Y.

Abstract
AIM: In prostate cancer (PCa), neuroendocrine differentiation (NED) is commonly observed in relapsing, hormone therapy-resistant tumours after androgen deprivation. However, the molecular mechanisms involved in the NED of PCa cells remain poorly understood. In this study, we investigated the expression of the neuroendocrine secretory protein secretogranin II (SgII) in PCa, and its potential involvement in the progression of this cancer as a granulogenic factor promoting NED.
METHODS: We have examined SgII immunoreactivity in 25 benign prostate hyperplasia and 32 PCa biopsies. In vitro experiments were performed to investigate the involvement of SgII in the neuroendocrine differentiation and the proliferation of PCa cell lines.
RESULTS: We showed that immunoreactive SgII intensity correlates with tumour grade in PCa patients. Using the androgen-dependent lymph node cancer prostate cells (LNCaP) cells, we found that NED triggered by androgen deprivation is associated with the induction of SgII expression. In addition, forced expression of SgII in LNCaP cells implemented a regulated secretory pathway by triggering the formation of secretory granule-like structures competent for hormone storage and regulated release. Finally, we found that SgII promotes prostate cancer (CaP) cell proliferation.
CONCLUSION: The present data show that SgII is highly expressed in advanced PCa and may contribute to the neuroendocrine differentiation by promoting the formation of secretory granules and the proliferation of PCa cells.

The LipoMag Kit kit is an association of DreamFect Gold and CombiMag reagents from OZ Biosciences. DreamFect™ Gold formulation is a lipofection reagent achieving high transfection efficiency and high transgene expression level. CombiMag is a Magnetofection™ reagent: versatile and improving transfection efficiency without toxicity. The LipoMag Kit combines both of the advantages of lipofection and magnetofection transfection technique to transfect all kind of cells with the best efficiency!