OZ Biosciences Blog

Thursday, October 30, 2014

Gene Silencing in MEF (Mouse Embryonic Fibroblast) using Lullaby transfection reagent

200 000 cells plated in 12-well dish were transfected with siRNA using Lullaby reagent for 48H.

This article demonstrates the high efficiency of Lullaby and the low toxicity of Lullaby, a transfection reagent from OZ Biosciences to induce gene silencing into Mouse Embryonic Fibroblasts.

article reference: DNA Repair, 26 September 2014

ATMIN is required for the ATM-mediated signaling and recruitment of 53BP1 to DNA damage sites upon replication stress

Luisa Schmidt, Marc Wiedner, Georgia Velimezi, Jana Prochazkova, Michel Owusu, Sabine Bauer, Joanna I. Loizou
Abstract
Unresolved replication intermediates can block the progression of replication forks and become converted into DNA lesions, hence exacerbating genomic instability. The p53-binding protein 1 (53BP1) forms nuclear bodies at sites of unrepaired DNA lesions to shield these regions against erosion, in a manner dependent on the DNA damage kinase ATM. The molecular mechanism by which ATM is activated upon replicative stress to localize the 53BP1 protection complex is unknown. Here we show that the ATM-INteracting protein ATMIN (also known as ASCIZ) is partially required for 53BP1 localization upon replicative stress. Additionally, we demonstrate that ATM activation is impaired in cells lacking ATMIN and we define that ATMIN is required for initiating ATM signaling following replicative stress. Furthermore, loss of ATMIN leads to chromosomal segregation defects. Together these data reveal that chromatin integrity depends on ATMIN upon exposure to replication-induced stress.

Lullaby is the ideal siRNA transfection reagent for gene silencing reaching up to 90% gene silencing. It protects siRNA from extracellular degradation and maintains high viability due to its bio-degradable properties.

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