Magnetic core shell nanoparticles delivery into U87 and MDA-MB-231 using Magnetofection

Delivery of magnetic nanoparticles into U87 and MDA-MB-231 was facilitated by Magnetofection using Magnetic plates from OZ Biosciences. 

article reference: ACS Nano. 2014 Sep 23;8(9):9379-87.

Core-shell nanoparticle-based Peptide therapeutics and combined hyperthermia for enhanced cancer cell apoptosis.

Shah BP, Pasquale N, De G, Tan T, Ma J, Lee KB.

Abstract

Mitochondria-targeting peptides have garnered immense interest as potential chemotherapeutics in recent years. However, there is a clear need to develop strategies to overcome the critical limitations of peptides, such as poor solubility and the lack of target specificity, which impede their clinical applications. To this end, we report magnetic core-shell nanoparticle (MCNP)-mediated delivery of a mitochondria-targeting pro-apoptotic amphipathic tail-anchoring peptide (ATAP) to malignant brain and metastatic breast cancer cells. Conjugation of ATAP to the MCNPs significantly enhanced the chemotherapeutic efficacy of ATAP, while the presence of targeting ligands afforded selective delivery to cancer cells. Induction of MCNP-mediated hyperthermia further potentiated the efficacy of ATAP. In summary, a combination of MCNP-mediated ATAP delivery and subsequent hyperthermia resulted in an enhanced effect on mitochondrial dysfunction, thus resulting in increased cancer cell apoptosis.

Magnetofection technology requires an appropriate magnetic field that magnetizes the nanoparticles in solution, forms a very strong gradient to attract the nanoparticles onto cells and covers all the surface of the culture dish. Therefore, optimized magnetic plates with tailored properties have been especially designed for Magnetofection.